Further studies on bilitranslocase,a plasma membrane protein involved in hepatic organic anion uptake |
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Authors: | Giancarlo Lunazzi Claudio Tiribelli Bruno Gazzin Gianluigi Sottocasa |
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Affiliation: | 1. Istituto di Chimica Biologica, Trieste Italy;2. Patologia Speciale Medica, Università di Trieste, Via Valerio 32, 34127 Trieste Italy |
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Abstract: | Bilitranslocase, a plasma membrane protein involved in bilirubin and other organic anion uptake by the liver, exhibits a high molecular weight (170 000) when isolated in the presence of deoxycholate. This value is decreased to approx. 100 000 if deoxycholate is not included in the isolation medium. Both preparations can be resolved into two kinds of subunit, α and β, of 37 000 and 35 500, respectively, by reduction with 2-mercaptoethanol and addition of sodium dodecyl sulfate. Under these conditions the two subunits are still capable of high-affinity sulfobromophthalein binding and, despite the presence of the detergent, may be isolated by preparative polyacrylamide gel electrophoresis still associated with the dye. It may be suggested that the physiological subunit composition of bilitranslocase is α2-β. |
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Keywords: | Bilitranslocase Sulfobromophthalein-binding protein Organic anion uptake (Liver plasma membrane) |
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