Co-operative binding of concanavalin A to A glycoprotein in lipid bilayers |
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Authors: | Nika V. Ketis Chris W.M. Grant |
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Affiliation: | Department of Biochemistry, The University of Western Ontario, London, Ontario N6A 5C1 Canada |
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Abstract: | Lectin-binding curves are reported for a concanavalin A receptor glycoprotein in lipid bilayers and intact cells. The results are consistent with previous studies of the structurally dissimilar transmembrane glycoprotein, glycophorin. High-affinity lectin binding to model membranes was influenced by the presence of apparently unrelated macromolecules, which we suggest is an example of receptor modulation by local interactions. Furthermore, high-affinity binding to the model membranes displayed characteristics, including positive cooperativity, similar to those seen with intact cells. |
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Keywords: | Concanavalin A Cooperativity Lipid bilayer Concanavalin A receptor Glycoprotein Lectin |
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