Ouabain-sensitive 86Rb(K) influx is linked to transepithelial Na transport in pig kidney cell line |
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| Authors: | Martin J Sanders Dayton S Misfeldt |
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| Institution: | Palo Alto Veterans Administration Medical Center and Stanford University, Stanford, CA 94305 U.S.A. |
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| Abstract: | The pig kidney cell line, LLC-PK1, exhibits rheogenic d-glucose coupled transepithelial Na+ transport that is inhibited by phlorizin. By measuring the difference in initial rates of influx of 86Rb+ with and without coupled Na+ transport, we can demonstrate an 86Rb+ uptake linked to Na+ transport. The simultaneous determination of phlorizin-inhibited Na coupled d-3H]glucose uptake and 86Rb+ influx allows calculation of an Na+/Rb+ stoichiometry that is consistent with an electrogenic Na+ for Rb+ exchange. |
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| Keywords: | Co-transport Ouabain sensitivity (Pig kidney) Hepes To whom correspondence should be addressed |
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