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Exogenous cytochrome c-dependent light-induced membrane potential generation in Rhodospirillum rubrum chromatophores
Authors:Nelly V. Kazennova  Vitaly D. Samuilov
Affiliation:Department of Microbiology, Moscow State University, Moscow 117234 U.S.S.R.
Abstract:Rhodospirillum rubrum chromatophores associated with a planar phospholipid macromembrane by bivalent cations in the presence of quinone, N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD) and ascorbate generate a transmembrane electrical potential difference in the light. Photoelectrical activity is also observed if chromatophores are preincubated with cytochrome c; the maximum values of responses are reached upon subsequent addition of ascorbate and menadion in the absence of bivalent cations and TMPD. The cytochrome c-dependent responses of the illuminated chromatophores are inhibited by Ca2+ and prevented by quinones. The possibility of cytochrome c (c2) translocation across the chromatophore membrane and the mechanism of charge transfer across the planar phospholipid membrane are discussed.
Keywords:Membrane potential  Chromatophore  Bacterial photosynthesis  Charge transfer  (R. rubrum)  TMPD  To whom reprint requests should be addressed
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