Cloning and Expression of a Bacillus thuringiensis (L1-2) Gene Encoding a Crystal Protein Active Against Glossina morsitans morsitans and Chilo partellus |
| |
| Authors: | Edward O Omolo M Diane James Ellie O Osir Jennifer A Thomson |
| |
| Institution: | (1) The International Center of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya , KE;(2) Microbiology Department, University of Cape Town, Private Bag, Rondebosch 7700, South Africa , ZA |
| |
| Abstract: | A local isolate of Bacillus thuringiensis,
designated L1-2, that is toxic to Chilo partellus was found to be
toxic to the adult tsetse fly, Glossina morsitans morsitans. The
δ-endotoxin crystals derived from the isolate gave a major protein band
with a molecular weight of Mr 130,000–140,000 on denaturing
polyacrylamide gel electrophoresis. The sequence of the cloned gene was found
to be similar to that of the B. thuringiensis subsp. kurstaki
HD-73 cryIA(c) gene, having one amino acid difference at position 148
and four additional DNA differences.
Received: 29 June 1996 / Accepted: 1 August 1996 |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
