Mouse macrophage hybridomas secreting a cytotoxic factor and interleukin 1 |
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| Authors: | Y Takeda H J Woo T Osawa |
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| Affiliation: | 1. Laboratory of Translational Cellular and Molecular Biomedicine, National Research Tomsk State University, Tomsk, 634050, Russia;2. Laboratory of Molecular Therapy of Cancer, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, 634009, Russia;3. Institute of Transfusion Medicine and Immunology, Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, University of Heidelberg, Mannheim, 68167, Germany;4. German Red Cross Blood Service Baden-Württemberg – Hessen, Mannheim, 68167, Germany;1. Shanghai Skin Disease Hospital, School of Medicine, Tongji University, 1278 Baode Road, Shanghai 200443, China;2. School of Medicine, Shanghai University, 99 Shangda Road, Shanghai 200444, China;3. Shanghai Engineering Research Center of Topical Chinese Medicine, 1278 Baode Road, Shanghai 200443, China;1. Department of Surgical Oncology, Breast Surgery, General Surgery,The First Hospital of China Medical University, Shenyang, China;2. Department of Cardiac Surgery,The First Hospital of China Medical University, Shenyang, China;3. Department of Laboratory Medicine, the Affiliated Hospital of Jiangnan University, Wuxi, China;4. Department of Pancreatic-Biliary Surgery, The First Hospital of China Medical University, Shenyang, China;5. Department of Ultrasound, The First Hospital of China Medical University, Shenyang, China |
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| Abstract: | Stable mouse macrophage hybridomas were produced by somatic cell fusion between proteose peptone-elicited peritoneal macrophages and NS-1 myeloma cells. Three cloned hybrid cell lines, designated as N/P-5-3, -6-2, and -7-1, exhibited typical macrophage-like morphology. Moreover, their macrophage characteristics were confirmed by the manifestation of intracellular nonspecific esterase, the detection of Mac-1 antigens and Fc-receptors on the cell surface, and the demonstration of phagocytic and antigen-presenting activities. Furthermore, these cell lines, stimulated with LPS, secreted considerable amounts of a cytotoxic factor and interleukin 1. Cultured cells of various tumor cell lines were sensitive to the cytotoxic factor, but normal thymocytes, spleen cells, and liver cells were not killed by the factor. |
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