绿色木霉ZY-1固态发酵产纤维素酶

利用筛选的绿色木霉ZY-1（Trichoderma viride ZY-1）固态发酵产纤维素酶，采用稻草和麸皮为底物，考察稻草与麸皮比例随发酵时间对产酶的影响。结果表明：底物中，在m（稻草）：m（麸皮）为0：5和1：4时，发酵48h，pH保持4．5左右，还原糖量急剧上升，胞外蛋白产量最低；仅以稻草作底物时，整个发酵过程中pH约为7，还原糖量最低，胞外蛋白产量较高而滤纸酶活、羧甲基纤维素酶（CMCase）和β-葡萄糖苷酶（β-Gase）酶活均较低；在m（稻草）：m（麸皮）为3：2时，发酵96h，滤纸酶活达最大值5．01U／g干曲；m（稻草）：m（麸皮）为1：4时，发酵96h，β-Gase酶活达最大值4．6U／g干曲；m（稻草）：m（麸皮）为4：1时，发酵72h，CMCase酶活达最大值6．01U／g干曲。因此，底物中存在适量的稻草和麸皮有利于Trichoderma viride ZY—1产纤维素酶。

Cellulase production by Trichoderma viride ZY-1 under solid-state fermentation

Cellulase was produced under solid-state fermentation by Trichoderma viride ZY-1 using wheat bran and straw as a substrate. The ratio effects of the rice straw to wheat bran on cellulase production was studied. The results showed that at higher concentration of wheat, bran, viz. the qualitative ratio of the rice straw to the wheat bran was 0：5 and 1 ： 4, after incubation for 48 h and pH remained at 4.5, the concentration of extracellular protein was the lowest, the concentration of reducing sugars was the highest, and activities of filter paper cellulase （ FPase）, carboxymethyl cellulase （ CMCase）, and β-glucosidase （β-Gase） were low. When rice straw was used as sole substrate and pH remained at 7, the concentration of reducing sugars was the lowest, but the concentrations of extracellular protein was high, the FPase, CMCase, and β-Gase activities were low. As the ratio of the rice straw to the wheat bran was 3： 2 and 1： 4, the activities of FPase and β-Gase reached the highest, i.e. 5.01 U/g dried substrate and 4.6 U/g dried substrate, respectively. As the ratio of the rice straw to the wheat bran was 4： 1, and fermentation time was 72 h, CMCase activity reached the highest at 6.01 U/g dried substrate.