Genetic analysis of kernel hardness in bread wheat using PCR-based markers |
| |
| Authors: | A A Galande R Tiwari J S S Ammiraju D K Santra M D Lagu V S Rao V S Gupta B K Misra S Nagarajan P K Ranjekar |
| |
| Institution: | (1) Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411 008, India e-mail: pkr@ems.ncl.res.in Fax: +91-20-5884032, IN;(2) Directorate of Wheat Research, Karnal 132 001, India, IN;(3) Agharkar Research Institute, Pune 411 004, India, IN |
| |
| Abstract: | In wheat, kernel hardness is a complex genetic trait involving various directly and indirectly contributing components such
as kernel hardness per se, protein content, hectolitre weight and 1,000-kernel weight. In an attempt to identify DNA markers associated with this trait,
100 recombinant inbred lines (RILs) derived from a cross between a hard grain land-race, NP4, and a soft grain variety, HB
208, were screened with 100 ISSR and 360 RAPD primers. Eighteen markers were assigned to seven linkage groups covering 223.6
cM whereas 11 markers remained unlinked. A multiple-marker model explained the percentage of phenotypic variation for kernel
hardness as 20.6%, whereas that for protein content, hectolitre weight and 1,000-kernel weight was 18.8%, 13.5% and 12.1%,
respectively. Our results indicate that phenotypic expression of kernel hardness is controlled by many QTLs and is interdependent
on various related traits.
Received: 25 July 2000 / Accepted: 24 November 2000 |
| |
| Keywords: | Wheat Kernel hardness (KH) Quantitative trait loci (QTL) RAPD ISSR Recombinant Inbred lines (RILs) |
| 本文献已被 SpringerLink 等数据库收录！ |
|
