Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. |
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| Authors: | D Botstein S C Falco S E Stewart M Brennan S Scherer D T Stinchcomb K Struhl R W Davis |
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| Affiliation: | 1. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, U.S.A.;1. Department of Biochemistry, Stanford University School of Medicine. Stanford, CA 94305 U.S.A. |
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| Abstract: | A system of biological containment for recombinant DNA experiments in Saccharomyces cerevisiae (Brewer's/Baker's yeast) is described. The principle of containment is sterility: the haploid host strains all contain a mating-type-non-specific sterile mutation. The hosts also contain four auxotrophic mutations suitable for selection for the various kinds of vectors used. All vectors are derivatives of pBR322 which can be selected and maintained in both yeast and Escherichia coli. The system has recently been certified at the HV2 level by the National Institutes of Health. |
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| Keywords: | HV2 pBR322 plasmid vectors 2-μ plasmid nonmating mutants |
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