黑盖木层孔菌发酵液多糖的分离纯化及活性研究

黑盖木层孔菌发酵液醇沉得胞外粗多糖(PNM)。气相色谱分析PNM为一杂多糖，单糖组成为：木糖、甘露糖、半乳糖、葡萄糖，质量比为0.05：0.63：0.31：1.00。PNM经冻融分级、Sevag法脱蛋白、Sepharose CL-6B柱层析得级分PNMⅠ，高效液相分析表明为单一级分，分子量约2.9×104。初步考察了PNM和PNMⅠ对小鼠离体淋巴细胞增殖的影响，二者都在400μg/ml时对淋巴细胞有极显著的增殖作用。

Studies on the isolation, purification and biological activity of the polysaccharides PNM from Phellinus nigiricans

The crude polysaccharide PNM was extracted from the submerged culture of Phellinus nigiricans by the method of ethanol precipitation. PNW is a heteropolysaccharide and GC analysis indicated that PNM consisted of xylise, mannose, galactose and glucose in the quality radio 0.05：0.63：0.31：1.00. PNM was purified by freeze thawing, methods of Sevag and preparation on Sepharose CL-6B column chromatography, it was PNMⅠ. HPLC analysis manifested that it was uniform fraction and the moleculer weight was about 29 throusand. Initially investigated the effects on the lymphocyte proliferation in vitro. The results were that both PNM and PNMⅠhad the most significant effects on the proliferation of mouse spleen cell in vitro at 400μg/ml.