ThePenicillium chrysogenum andAspergillus nidulans wetA developmental regulatory genes are functionally equivalent |
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Authors: | Rolf A. Prade and William E. Timberlake |
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Affiliation: | (1) Dept. of Genetics, University of Georgia, 30602-7223 Athens, GA, USA;(2) Present address: Myco Pharmaceuticals Inc., Building 300, One Kendall Square, 02139 Cambridge, MA |
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Abstract: | Aspergillus nidulans andPenicillium chrysogenum are related fungi that reproduce asexually by forming multicellular conidiophores and uninucleate conidia. InA. nidulans, spore maturation is controlled by thewetA (AwetA) regulatory gene. We cloned a homologous gene (PwetA) fromP. chrysogenum to determine if spore maturation is regulated by a similar mechanism in this species. ThePwetA andAwetA genes are similar in structure and functional organization. The inferred polypeptides share 77% overall amino acid sequence similarity, with several regions having > 85% similarity. The genes also had significant, local sequence similarities in their 5 flanking regions, including conserved binding sites for the product of the regulatory geneabaA.PwetA fully complemented anA. nidulans wetA deletion mutation, demonstrating thatPwetA and its 5 regulatory sequences function normally inA. nidulans. These results indicate that the mechanisms controlling sporulation inA. nidulans andP. chrysogenum are evolutionarily conserved. |
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Keywords: | Aspergillus nidulans Penicillium chrysogenum wetA Regulatory gene Sporulation |
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