铜绿假单胞菌生物膜悬液和藻酸盐对小鼠巨噬细胞吞噬功能的影响

目的探讨铜绿假单胞菌（PA）生物膜和藻酸盐成分对小鼠巨噬细胞吞噬功能的影响。方法用具有生物膜成分的PA悬液分别感染肺部巨噬细胞缺乏小鼠和正常小鼠,比较组织中的细菌数量。提取小鼠腹腔巨噬细胞,藻酸盐作用后加入PA悬液,测定巨噬细胞对细菌的吞噬率。巨噬细胞经不同浓度的藻酸盐作用后,中性红法检测巨噬细胞吞噬能力。结果巨噬细胞缺乏组和对照组肺部组织的细菌数量分别为（4.16±3.36）×10^5/ml和（5.15±1.92）×10^5/ml,t=0.7211,P=0.483。生物膜细菌组的巨噬细胞吞噬率与对照组的吞噬率分别为（13.82±4.71）%和（42.73±11.00）%,Q=12.3231,P〈0.01。表明生物膜细菌组比对照组更能抵抗巨噬细胞的吞噬。加藻酸盐组的巨噬细胞吞噬率与对照组的吞噬率分别为（22.91±6.20）%和（42.73±11.00）%,Q=8.4465,P〈0.01。表明加藻酸盐组比对照组更能抵抗巨噬细胞的吞噬。当藻酸盐浓度为0、25、50、75、100、125、150μg/ml时,以吸光度A（540nm）值表示其巨噬细胞吞噬中性红的能力分别为：0.271±0.044、0.456±0.062、0.445±0.061、0.551±0.065、0.210±0.053、0.186±0.026、0.195±0.025。当藻酸盐≤75μg/ml时巨噬细胞吞噬中性红的能力增强,与0μg/ml组相比P〈0.05;当藻酸盐〉75μg/ml时巨噬细胞吞噬中性红的能力降低,与0μg/ml组相比P〈0.05。结论巨噬细胞有阻止PA入侵的作用。PA生物膜可以抑制巨噬细胞的吞噬。PA生物膜藻酸盐成分在〈75μg/ml时促进巨噬细胞的吞噬,而在较大剂量时抑制巨噬细胞的吞噬功能。

The influence of Pseudomonas aeruginosa biofilm suspension and alginate on macrophage phagocytosis function in mouse

Objective To explore the influence of Pseudomonas aeruginosa （PA） biofilm and alginate on macrophage phagocytosis function in mouse. Methods The bacterial biofilm suspension of PA was used to infect maerophage-de- pleted mouse and control mouse to explore the influence of biofilm on maerophage. The maerophage was isolated from mouse and added with alginate inside, then the phagocytosis rate was determined. The capability of maerophage＇s phagocytosis was detected by neutral red method. Results The bacterial count in maerophage-depleted mouse compared to the control group was （4.16 ± 3.36） ×10^5/ml to （5.15 ±1.92） ×10^5/ml, t = 0. 7211, P = 0. 483. The phagocytosis rate in PA biofilm group compared to the control group was （ 13.82 ± 4.71 ） % to （42.73 ± 11.00） %, Q = 12.3231,P 〈 0. 01. This suggested that the PA biofilm group can be more resistant to maerophage phagocytosis compared to the control group. The phagocytosis rate in alginate group compared to the control group was （22.91 ± 6.20 ） % to （42.73 ± 11.00） %, Q = 8. 4465, P 〈 0.05. This showed that the alginate group can also be more resistant to maerophage phagocytosls compared to the control group. When the concentration of alginate were 0,25,50,75,100,125 and 150 μg/ml the absorbanee A（ 540 nm） which represents the capability of maerophage phagocytosing neutral red, were （ 0.271 ± 0. 044 ）, （ 0. 456 ± 0. 062 ） , （ 0. 445 ± 0.061 ） , （0.551 ± 0.065 ）, （0.210 ± 0. 053 ）, （0.186 ± 0. 026 ） and （ 0. 195 ± 0. 025 ） respectively. When the alginate＇ s concentra- tion ≤75 μg/ml, the capability of maerophage phagocytosing neutral red strengthened （ P 〈 0.05 ） , compared to the 0 μg/ml group; When the alginate＇s concentration 〉 75μg/ml, the capability of maerophage phagoeytosing neutral red decreased（ P 〈 0.05 ） , compared to the 0 μg/ml group. Conclusion Maerophage can prevent the invasion of PA. PA biofilm can inhibit maerophage phagoeytosis. Alginate can pro