Micropropagation and slow growth conservation of cardamom (<Emphasis Type="Italic">Elettaria cardamomum</Emphasis> Maton) |
| |
Authors: | Rishi K Tyagi Rajni Goswami Rajkumari Sanayaima Rakesh Singh Rajesh Tandon Anuradha Agrawal |
| |
Institution: | (1) Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, New Delhi, 110012, India;(2) NRC on DNA Fingerprinting, National Bureau of Plant Genetic Resources, New Delhi, 110012, India;(3) Department of Botany, University of Delhi, Delhi, 110007, India |
| |
Abstract: | Cardamom (Elettaria cardamomum Maton) has great commercial value as a spice crop in India. A one-step protocol for direct regeneration of plants and in vitro conservation by slow growth method has been developed. A maximum of 6.5 shoots/culture were obtained in 2 mo or 15.1 shoots/culture
in 4 mo on Murashige and Skoog (Physiol Plant 15:473–497, 1962) medium (MS) + 5 μM benzylaminopurine gelled with 0.7% agar (micropropagation medium). Rooting also occurred simultaneously
on the same medium. Using one shoot tip or nodal explant, about 30,375 plants can be regenerated in a year on the micropropagation
medium. In vitro conservation by slow growth method was achieved on 1/2 MS (major salts) + 5 μM BAP + 0.7% agar (conservation medium); about
70% of the cultures survived up to 18 mo at 25 ± 2°C. Successful regrowth of plants on micropropagation medium was obtained
by culturing nodal explants excised from 18-mo-old conserved plants. Some 96% of the plants survived the hardening treatment
and grew normally in a greenhouse. If 24 cultures are conserved on the conservation medium, it is possible to regenerate at
least 750 plants by using explants derived from 70% of the surviving shoots and culturing the same in micropropagation medium
for 4 mo. These plants may be used for planting or as a source of explants for the next conservation cycle. On the basis of
20 random amplified polymorphic DNA and 13 inter-simple sequence repeat primers analyses, no significant reproducible variation
was detected among the in vitro-conserved plants compared with the mother plants. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|