肠缺血再灌注时肠内给予不同营养物对肠粘膜ATP含量的影响

将SD大鼠分组 ,先制作空肠袋 ,分别向袋内注射不同营养物 :10mmol/L丙氨酸 ,10mmol/L葡萄糖 ,10mmol/L甘露醇或 5mmol/L丙氨酸 +5mmol/L葡萄糖的混合液 ,用动脉夹阻断肠系膜上动脉血流 6 0min后 ,再恢复灌流 6 0min。分别于阻断血流 6 0min和恢复灌注 6 0min测定肠粘膜ATP含量。研究结果显示 ,缺血再灌注能显著降低肠粘膜ATP含量 ,给予丙氨酸或葡萄糖 /丙氨酸混合液使肠粘膜ATP含量进一步降低 (P <0 .0 1) ,而给予葡萄糖能显著增加肠粘膜ATP含量 (P <0 .0 1)。结论 :缺血再灌注过程中 ,肠内给予葡萄糖能改善肠粘膜ATP含量 ,对缺血再灌注损伤的肠道提供保护作用

The Effects of Specific Enteral Nutriets on Mucosal ATP Content During Gut Ischemia/Reperfusion

To determine if the specific nutrients are associated with alterations in mucosal ATP contents during gut I/R, Sprague Dawley rats had a jejunal sacs filled with different nutrients(5mmol/L alanine+5mmol/L glucose, 10mmol/L glucose, 10mmol/L alanine, or 10mmol/L mannitol), followed by 60 minutes of superior mesenteric artery occlusion and 60 minutes of reperfusion. Mucosal ATP contents were determined at either 60 minutes ischemia or 60 minutes of reperfusion. ATP levels were decreased after I/R. Intraluminal alanine alone or alanine/glucose further reduced ATP content, but ATP levels were increased during both ischemia and reperfusion when intraluminal glucose was present as compared to intraluminal alanine alone or alanine/glucose. The results indicated that intraluminal glucose could augment mucosal ATP levels during gut I/R and protect gut from ischemia and reperfusion injury.