| 响应面法优化两歧双歧杆菌发酵培养基 |
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| 引用本文： | 杜少平,梁淑娃,杨冠东,蓝碧锋,彭中健.响应面法优化两歧双歧杆菌发酵培养基[J].生物技术通报,2008(Z1). |
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| 作者姓名： | 杜少平 梁淑娃 杨冠东 蓝碧锋 彭中健 |
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| 作者单位： | 广州市微生物研究所,广州,510250 |
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| 基金项目： | 广州市科技局成果推广课题资助(2006C13G0071) |
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| 摘 要： | 根据两歧双歧杆菌的营养需要和生长特性,采用响应面分析法对两歧双歧杆菌的培养基进行优化研究。先用Plackett-Burman设计法实验确定重要因素,再用最陡爬坡实验法确定因素水平,最后用响应面分析方法求得的最佳培养基配方为经优化的发酵培养基配方为:酪蛋白胨1.0%,大豆蛋白胨0.5%,酵母膏1.63%,半胱氨酸盐酸盐0.0076%,低聚果糖0.13%,葡萄糖0.5%,K2HPO4 0.2%。用此优化的发酵培养基培养两歧双歧杆菌,活菌数可高达7.8×10~9 cfu/ml。
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| 关 键 词： | 两歧双歧杆菌 响应面分析法 培养基优化 |
Optimization of Bifutobacterium bifidum Fermentation Medium Using Response Surface Analysis |
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| Du Shaoping,Liang Shuwa,Yang Guandong,Lan Bifeng,Peng Zhongjian.Optimization of Bifutobacterium bifidum Fermentation Medium Using Response Surface Analysis[J].Biotechnology Bulletin,2008(Z1). |
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| Authors: | Du Shaoping Liang Shuwa Yang Guandong Lan Bifeng Peng Zhongjian |
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| Institution: | Guangzhou Microbe Reseach Institute;Guangzhou 510250 |
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| Abstract: | Response surface methodology of SAS software was used to optimize fermentation medium for Bifidobacterium bifidum in this paper.First,used Plackett-Burman design to select the most important nutrition factors which affecting the growth of Bifidobacterium bifidum.Then,the steepest ascent was used to approach the optimal region of the medium composition.The optimal concentrations of yeast extract,oligofructose,cysteine muriate were determined by a central composite design and response surface analysis.And the... |
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| Keywords: | Bifidobacterium bifidum Response surface analysis Fermentation medium optimization |
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