Spore-displayed streptavidin: a live diagnostic tool in biotechnology |
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| Authors: | Kim June-Hyung Lee Chang-Soo Kim Byung-Gee |
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| Institution: | Institute of Molecular Biology and Genetics, School of Chemical Engineering, Seoul National University, Republic of Korea. |
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| Abstract: | Streptavidin, which is one of the most widely used proteins in biotechnological application field and is active only in tetrameric form, was surface expressed on the surface of Bacillus subtilis spore. Spore coat protein of B. subtilis, CotG, was used as an anchoring motif to display streptavidin. FACS using anti-streptavidin antibody was used for the verification of surface localization of expressed CotG-streptavidin fusion protein. FACS and dot-blot were used for the verification of biological activity of displayed streptavidin with FITC-labeled biotin. |
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| Keywords: | Bacterial surfce display Streptavidin Bacillus subtilis Spore |
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