Nature of DNA-bound fatty acids in Pseudomonas aurantiaca |
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Authors: | Zhdanov Renat I Shmyrina Anastasia S Zarubina Tatyana V Mulyukin Andrey L El-Registan Galina I Haupt Nicole Kraus Angelika Lorenz Wilhelm |
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Institution: | Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russian Federation. zrenat@hotmail.com |
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Abstract: | The existence of Pseudomonas aurantiaca DNA-bound fatty acids and lipids is presented in this work. The isolation of DNA was carried out by two different procedures, namely, phenol and detergent-based phenol isolation in order to prove the presence of DNA-bound lipids. The lipid content of DNA is expressed in terms of fatty acid profile. A high level of 16:0, 18:0 and 18:1 is characteristic for tightly bound DNA lipids. On the other hand, the fatty acids such as 14:1, iso14:0 and iso16:0 are found in trace amounts only in DNA lipid fraction, but these fatty acids are not found in the whole-cell lipids. Absolutely no 3-hydroxy fatty acids were found in DNA lipids. However, both C16 and C18 species represent the main fatty acids of whole-cell and DNA-bound lipids. The presence of DNA-bound lipids even under tough treatment of DNA allows to conclude that these lipids represent a special pool among cellular lipids. |
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Keywords: | genomic DNA DNA-bound fatty acids FAME analysis fatty acid profile |
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