Idiotype-specific T helper clones recognize a variable H chain determinant. |
| |
Authors: | Y Saeki J J Chen L F Shi Y Okuda H K?hler |
| |
Institution: | IDEC Pharmaceuticals Corporation, La Jolla, CA 92037. |
| |
Abstract: | Previously we described a Th clone specific for a regulatory idiotype on 3A4, an anti-Id mAb that mimics a murine L1210/GZL tumor-associated Ag. In our studies, we determined the molecular target on the stimulating anti-Id antibody that is recognized by the Th clone. The Th clone responds with proliferation to the H chain of 3A4 but not to the L chain. Furthermore, the 3A4 chain stimulates this Th clone more efficiently than either the intact 3A4 or the Fab fragments, and the presentation by APC of the H chain is more resistant to chloroquine treatment than the presentation of the intact 3A4 molecule. These results suggest that regulatory T cells "see" their target idiotopes as linear sequence determinants present on isolated Ig chains, and show that this might have biologic advantages with respect to the mechanism of Ag presentation. |
| |
Keywords: | |
|
|