Detection and identification of gibberellins in Sitka spruce (Picea sitchensis) of different ages and coning ability by bioassay, radioimmunoassay and gas chromatography – mass spectrometry |
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Authors: | Thomas Moritz J Julian Philipson Per Christer Odén |
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Institution: | Dept of Forest Genetics and Plant Physiology, Swedish Univ. of Agricultural Sciences, S-901 83 Umea, Sweden;Forestry Commission Northern Research Station, Roslin Midlothian, EH25 9SY UK;Dept of Forest Genetics and Plant Physiology, Swedish Univ. of Agricultural Sciences. S-901 83 Umea, Sweden. |
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Abstract: | Gibberellins Al (GA1), GA3, GA4, GA9, and after enzymatic hydrolysis of GA-conjugate-like fractions, GA9 and GA15, were identified in shoots of Sitka spruce Picea sitchensis (Bong.) Carr.] of different ages by combined gas chromatography-mass spectrometry (GC-MS). The purification and separation of the GAs involved the use of reverse phase and normal phase high performance liquid chromatography (HPLC). The Tan-ginbozu dwarf rice bioassay and binding to antibodies raised against GA1, GA4 and GA9 were used for detection of GA-like substances. The qualitative differences between the three ages of plant material were the presence of GA3 and GA1 in the 48-year-old material and the absence of detectable amounts of GA4 in the same material. This indicates a difference in GA metabolism which may reflect the difference in ability to form reproductive buds. |
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Keywords: | Bioassay flowering gas chromatography – mass spectrometry gibberellins Picea sitchensis radioimmunoassay Sitka spruce |
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