Analysis of Secondary Metabolites of Microscopic Fungi of the Genus Penicillium by Chromatographic Techniques |
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| Authors: | Zelenkova N F Vinokurova N G Arinbasarov M U |
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| Institution: | (1) Russian Academy of Sciences, Skryabin Institute of the Biochemistry and Physiology of Microorganisms, pr. Nauki 5, Pushchino, Moscow oblast, 142290, Russia |
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| Abstract: | Combinations of various systems of thin-layer chromatography and high-performance liquid chromatography (HPLC) were efficient in analyzing 39 nitrogen-containing secondary metabolites (alkaloids) produced by 12 strains of microscopic fungi of the genus Penicillium. Chromatographic mobility of alkaloids on Silufol plates was determined in the following systems: (a) chloroform, methanol, and 25% NH4OH (90 : 10 : 1, 90 : 10 : 0.1, or 80 : 20 : 0.2); (b) chloroform and acetone (9 : 1); and (c) ethyl acetate, methanol, and 25% NH4OH (85 : 15 : 10); staining was performed using Ehrlich's reagent. Conditions for separation of clavine alkaloids by HPLC on Spherisorb ODS-2 and Supelcosil LC-18 columns (gradient elution) were optimized. Retention values of 22 alkaloids were compared to those of agroclavine and roquefortine. |
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