A map of rye chromosome 4R with cytological and isozyme markers |
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Authors: | C Benito F Llorente N Henriques-Gil F J Gallego C Zaragoza A Delibes A M Figueiras |
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Institution: | (1) Department of Genetics, Faculty of Biology, University Complutense, E-28040 Madrid, Spain;(2) Laboratory of Genetics, Centro de Enseñanza Superior San Pablo, CEU, Monteprincipe, Boadilla del Monte, E-28660 Madrid, Spain;(3) Department of Biochemistry and Molecular Biology, E.T.S. Ingenieros Agrónomos-UPM, E-28040 Madrid, Spain |
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Abstract: | The progeny of two crosses between a structural heterozygote for a reciprocal translocation (4RL/5RL) and a homozygote for the standard chromosome arrangement and of four crosses between standard chromosome homozygotes were analysed in rye (Secale cereale L. cv Ailés ) for the electrophoretic patterns of five different leaf and endosperm isozymes (LAP, PGM, NDH, ADH and EPER). The presence or absence of the quadrivalents at metaphase I (MI) was also tested. Loci Adh-1, Pgm-1 and Ndh-1 were located on chromosome arm 4RS, and locus Eper-1 on chromosome arm 4RL. Locus Lap-2 was located on the 4RS chromosome arm. The estimated distances among the different linked loci support the following gene order: Eper1¨ (breakpoint-centromere)¨Lap-2¨ ¨Adh-1 ¨Pgm-1¨Ndh-1. These results provide evidence for the chromosomal location of Lap-2 locus on chromosome arm 4RS in cv Ailés . A high negative interference was detected between the zones delimited by centromere and Lap-2, and Lap-2 and Pgm-1 in plants with the 4RL/5RL translocation.Abbreviations LAP
leucine aminopeptidase
- PGM
phosphoglucomutase
- NDH
NADH dehydrogenase
- ADH
alchohol dehydrogenase
- EPER
endosperm peroxidase |
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Keywords: | Isozymes Translocation Cytogenetic maps Chromosome 4R Rye |
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