Isolation of Neospora caninum from the brain of a pregnant sheep. |
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| Authors: | T Koyama Y Kobayashi Y Omata M Yamada H Furuoka R Maeda T Matsui A Saito T Mikami |
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| Institution: | Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Japan. |
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| Abstract: | Neospora caninum was isolated from the brain of a naturally infected pregnant sheep by inoculation of immunodeficient mice with a homogenate of the brain tissue. The ewe showed no clinical signs. Tachyzoites were observed in the tissues of the nu/nu mice injected with the brain tissue homogenate and the diagnosis was confirmed by immunohistochemical staining with anti-N. caninum antibodies and by detecting N. caninum-specific DNA by polymerase chain reaction. |
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