Mithramycin and DIPI: A pair of fluorochromes specific for GC- and AT-rich DNA respectively |
| |
Authors: | W Schnedl M Breitenbach A -V Mikelsaar G Stranzinger |
| |
Institution: | (1) Histologisch-Embryologisches Institut, University of Vienna, Schwarzspanierstraße 17, A-1090 Wien, Austria;(2) Institut für Allgemeine Biochemie der Universität Wien, Austria;(3) Lehrstuhl für Tierzucht der Technischen Universität München, Freising-Weihenstephan |
| |
Abstract: | Summary The AT specificity of the fluorochromes DIPI and DAPI and the GC specificity of mithramycin are evidenced by observations in human, mouse, and bovine chromosomes. DIPI and DAPI produce a pattern similar to Hoechst 33258 in all three species, whereas mithramycin results in a reverse pattern. The AT-rich centromeric heterochromatin in mouse is brilliantly stained by DIPI or DAPI and remains nearly invisible after mithramycin staining. In the GC-rich centromeric heterochromatin of cattle the opposite behavior is observed. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|