Stereospecific deuteration of 2-deoxyerythrose 4-phosphate using 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase |
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| Authors: | Williamson Rachel M Pietersma Amy L Jameson Geoffrey B Parker Emily J |
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| Affiliation: | Institute of Fundamental Sciences, Massey University, Palmerston North 5301, New Zealand. |
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| Abstract: | Racemic 2-deoxyerythrose 4-phosphate was synthesized and one enantiomer of this compound was found to be a substrate for Escherichia coli 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, the first enzyme of the shikimate pathway. When the reaction was carried out in deuterium oxide, an enzyme-catalyzed regio- and stereoselective incorporation of deuterium into the product was observed. |
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