dbEST-derived SSR markers in sea urchin (Hemicentrotus pulcherrimus) |
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| Authors: | Xiuli Wang Teng Feng Lei Yang Chengcheng Liu Xiangying Meng Xuemei Qiu |
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| Institution: | (1) College of Life Sciences and Biotechnology, Dalian Fisheries University, 52th, Heishijiao Street, Shahekou District, Dalian, 116023, China;(2) Marine Drug and Food Institute, Ocean University of China, Qingdao, 266003, China;(3) Department of Chemistry, Tsinghua University, Beijing, 100084, China |
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| Abstract: | Sea urchin is an important model organism for evolutionary biology, embryology, and gene regulation study. We developed and
evaluated simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) of Strongylocentrotus
purpuratus and Hemicentrotus pulcherrimus. Characteristics of nine EST-SSR loci were investigated using 41 Hemicentrotus pulcherrimus individuals. The number of alleles per locus ranged from two to five. The observed heterozygosity ranged from 0.122 to 0.7805, while the
expected heterozygosity ranged from 0.4472 to 0.7696. These loci and markers will be useful for population genetics and systemic
evolution among species of sea urchin. |
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| Keywords: | EST SSRs Sea urchin Hemicentrotus pulcherrimus Strongylocentrotus purpuratus |
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