Enzyme activity in dialkyl phosphate ionic liquids |
| |
Authors: | Thomas Marie F Li Luen-Luen Handley-Pendleton Jocelyn M van der Lelie Daniel Dunn John J Wishart James F |
| |
Affiliation: | Department of Chemistry, Brookhaven National Laboratory, P.O. Box 5000, Upton, NY 11973, USA. mthomas@bnl.gov |
| |
Abstract: | The activity of four metagenomic enzymes and an enzyme cloned from the straw mushroom, Volvariella volvacea were studied in the following ionic liquids, 1,3-dimethylimidazolium dimethyl phosphate, [mmim][dmp], 1-ethyl-3-methylimidazolium dimethyl phosphate, [emim][dmp], 1-ethyl-3-methylimidazolium diethyl phosphate, [emim][dep] and 1-ethyl-3-methylimidazolium acetate, [emim][OAc]. Activity was determined by analyzing the hydrolysis of para-nitrobenzene carbohydrate derivatives. In general, the enzymes were most active in the dimethyl phosphate ionic liquids, followed by acetate. Generally speaking, activity decreased sharply for concentrations of [emim][dep] above 10% v/v, while the other ionic liquids showed less impact on activity up to 20% v/v. |
| |
Keywords: | Ionic liquid Cellulases Enzyme activity Enzymatic hydrolysis |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|