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Tomato spotted wilt virus (TSWV) infection of Physcomitrella patens gametophores
Authors:Hühns  Saskia  Bauer  Christel  Buhlmann  Sven  Heinze  Cornelia  von Bargen  Susanne  Paape  Martina  Kellmann  Jan-Wolfhard
Institution:(1) Fachbereich Biowissenschaften, Abteilung Biochemie, Universität Rostock, Albert-Einstein-Straße 3, D-18059 Rostock, Germany;(2) Institut für Angewandte Botanik, Abteilung Pflanzenschutz, Universität Hamburg, Ohnhorststraße 18, D-22609 Hamburg, Germany;(3) Present address: Institut für Immunologie, Universität Rostock, Schillingallee 70, 18055 Rostock, Germany
Abstract:Following mechanical inoculation of the moss Physcomitrella patens (Hedw.) B.S.G. with Tomato spotted wilt virus (TSWV), the virus encoded N nucleocapsid protein was detected in gametophores harvested 11 and 29 dpi and the non-structural NSm movement protein was observed 29 dpi. The detection of both viral proteins presumes that P. patens could serve as a new lab–host for TSWV, allowing reverse genetics by gene targeting to elucidate the role of specified molecular virus–host interactions.
Keywords:Bryophyta  Bunyaviridae  movement protein  nucleocapsid protein  Physcomitrella patens  plant virus  TSWV
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