Time-resolved spectroscopy of the blue fluorescence of spinach leaves |
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Authors: | Yves Goulas Ismael Moya Guido Schmuck |
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Institution: | (1) Départment de Bioclimatologie, Institut National de la Recherche Agronomique, 84140 Montfavet, France;(2) Laboratoire pour l'Utilisation du Rayonnement Electromagnetique, Université de Paris XI, 91405 Orsay, France;(3) Ispra Establishment, Joint Research Center, 21020 Varese, Italy |
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Abstract: | When excited by ultraviolet radiation, leaves of a great number of species of higher plants exhibit emission of blue fluorescence, comparable in intensity to the red emission of chlorophyll. The fluorescence decay of the blue emission of spinach leaves recorded by single photon counting techniques is decomposed into exponential components and it is shown that at least three different components are present. The lifetime of the three components does not show significant variations with the excitation or emission wavelengths. The excitation and emission spectra of each component were determined. The nature of the chemical compounds which cause this emission is discussed in relation to these spectra. |
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Keywords: | algae blue fluorescence ferulic acid flavonoid photon counting polyphenolics spinach time-resolved fluorescence |
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