Cross-linked stem bromelain: A more stabilized active preparation |
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Authors: | Tehseen Anwar Basir Ahmad Hina Younus |
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Institution: |
a Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India |
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Abstract: | Cross-linking of the protease stem bromelain (bromelain) with 0.25 and 1.25% glutaraldehyde (GTA) results in the formation of a large molecular mass, multimeric and soluble aggregate having comparable activity to the unmodified bromelain. Both 0.25 and 1.25% GTA cross-linked (CL) bromelain preparations were more stable against urea, guanidine hydrochloride (GdnHCl) and temperature-induced inactivation, and exhibited slightly better storage stability compared to the unmodified protease. Such a high molecular weight, soluble, active and stable preparation may be useful in industry, i.e. in the textile industry for improving the properties of a fabric without loss of fabric strength and shape. |
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Keywords: | Cross-linking glutaraldehyde proteases stability stem bromelain |
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