Lysosomal enzymes produced by immobilized Tetrahymena thermophila |
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Authors: | Thomas Kiy Arno Tiedtke |
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Institution: | (1) Zoologisches Institut der Universität Münster, Schlossplatz 5, D-4400 Münster, Federal Republic of Germany |
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Abstract: | Summary The ciliated protozoon Tetrahymena thermophila was immobilized for production of secreted lysosomal enzymes in two ways. Cells entrapped in solid Ca-alginate spheres survived but were unable to grow and multiply. However, when encapsulated in hollow Ca-alginate spheres Tetrahymena multiplied well, reaching 0.9 × 107 cells/ml. These immobilized cells secreted large amounts of lysosomal enzymes when the medium was changed daily. This system was transferred to a reactor scale using a conical bubble column reactor for semicontinuous cultivation of the encapsulated cells. Under these conditions -glucosidase, -glucosidase, -hexosaminidase and acid phosphatase were produced for at least 4 weeks. The hollow spheres were stable for 3 months and contained living and secreting Tetrahymena cells during this time. Immobilized T. thermophila cells can thus serve as a good source for production of commercially interesting enzymes.
Offprint requests to: A. Tiedtke |
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