Optical studies on the structure of hemoglobin messenger ribonucleic acid |
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| Authors: | M. Brentani J.M. Salles K. Zinner A. Faljoni R. Brentani |
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| Affiliation: | 1. Laboratório de Oncologia Experimental, Faculdade de Medicina Universidade de São Paulo, Caixa Postal 8100, São Paulo, Brasil.;2. Departamento de Bioquímica, Instituto de Química, Caixa Postal 20780, São Paulo, Brasil. |
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| Abstract: | The secondary structure of hemoglobin mRNA (HbmRNA has been investigated by optical rotatory dispersion (ORD), circular dichroism (CD) and ultraviolet absorbance spectrophotometry. The dependence on temperature or reaction with formaldehyde of the CD and absorbance are characteristic of a structure with substantial base pairings at 20°C. The presence of Cotton effects in one of the major ultraviolet absorption bands indicates a highly ordered secondary structure. The UV hyperchromism on thermal denaturation is consistent with a value of 58% double helical content. |
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| Keywords: | SDS sodium dodecyl sulfate ORD optical rotatory dispersion CD circular dichroism |
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