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Glycosylation of rat liver cytochrome b5 on the ribosomal level
Authors:Åke Elhammer  Gustav Dallner  Tsuneo Omura
Institution:1. Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, Stockholm, Sweden;2. Department of Pathology at Huddinge Hospital, Karolinska Institutet, Stockholm, Sweden;3. Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan
Abstract:The distribution and site of synthesis of cytochrome b5 was studied by antibody precipitation of the enzyme labeled invivo. The enzyme is present in rough and smooth microsomes, Golgi and outer mitochondrial membranes. The cytochrome is synthesized only on bound ribosomes, where glucosamine and galactose moieties are also added. The enzyme seems to be devoid of mannose and sialic acid residues.
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