Reversible inhibition of adenylate cyclase activity of rat brain caudate nucleus by oxidized glutathione |
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| Authors: | Akemichi Baba Eibai Lee Toshio Matsuda Tetsuroh Kihara Heitaroh Iwata |
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| Institution: | Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan |
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| Abstract: | Basal and dopamine-stimulated adenylate cyclase (EC 4.6.1 1.) activities were strongly inhibited by GSSG, but not by GSH. Adenylate cyclase that had been inactivated by GSSG was reactivated by incubation with various sulfhydryl compounds including GSH. Formation of mixed disulfides by reaction between GSSG and protein-SH groups increased on incubation with GSSG and returned to the normal level on subsequent incubation with DTT. |
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| Keywords: | EGTA DTT dithiothreitol |
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