The relation between fatty acid mobilization and contractility in the isolated,perfused rat heart |
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Authors: | H. Stam W.C. Hülsmann |
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Affiliation: | Thoraxcenter & Dept. of Biochemistry I, Erasmus University Rotterdam, The Netherlands |
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Abstract: | The contractility of hearts from normal fed rats is decreased by 70% during perfusion with 50 μM chloroquine, which is a potent inhibitor of endogenous lipolysis. In triacylglycerol-rich hearts, obtained by feeding rats rapeseed-oil, chloroquine depresses lipolysis much less, while contractility was found to be inhibited only 30%. In both groups of hearts the effect of chloroquine was decreased by adding fatty acids, prostaglandin E1, the ionophore X-537A or more Ca2+ to the perfusion fluid. Norepinephrine and glucagon also stimulate chloroquine-depressed hearts. The conclusion is therefore reached that fatty acids act as Ca2+-vehicles in heart cells and that chloroquine, by inhibiting lipolysis, decreases Ca2+-transport by lowering unesterified fatty acid levels. |
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