Intracellular location of human fibroblast interferon messenger RNA |
| |
Authors: | Sergio L Abreu FCarter Bancroft |
| |
Institution: | Interferon Laboratories, Memorial Sloan-Kettering Cancer Center New York, N.Y. 10021 USA |
| |
Abstract: | Human diploid fibroblast (FS-4) cells were induced to produce interferon mRNA by exposure to poly(rI)·poly(rC) plus cycloheximide. The intracellular location of interferon mRNA was investigated by differential centrifugation of the cytoplasm into a membrane (pellet) and a free (supernatant) fraction, followed by injection of mRNA isolated from either fraction into oocytes. When translation in FS-4 cells was prevented, most (85–90%) of the interferon mRNA activity was found in the free fraction. However, when translation was permitted, most (80–95%) of the interferon mRNA activity was found in the membrane fraction. These results are consistent with the predictions of the “signal hypothesis” (Blobel and Dobberstein, J. Cell Biol. 1975, :835) for secretory proteins. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|