The folding of pancreatic elastase: Independent domain refolding and inter-domain interaction

The role of domains in the refolding of elastase, a two domain protein, was investigated. It has been demonstrated that fragment 126–245, corresponding to one of the two domains, is able to refold independently. Moreover, the in vitro complementation of the two domains lead to a molecule having the overall conformation of the native protein and only a weak but significant activity.