Identification and synthesis of a tripeptide in ECUM fluid of an upemic patient

An unidentified ninhydrin and Pauly reaction positive substance of basic nature was found in the ECUM fluid of an uremic patient. This substance was isolated from ECUM fluid by the methods of ultrafiltration method and gel-filtration, and identified as H-His-Gly-Lys-OH by amino acid analysis, manual Edman degradation method and physical constants and analytical data of synthetic tripeptide.