Inside-out membrane vesicles isolated from spinach thylakoids |
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Authors: | Bertil Andersson Hans-Erik Åkerlund |
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Affiliation: | Dept. of Biochemistry 1, University of Lund, Chemical Center, P.O. Box 740, S-220 07 Lund Sweden |
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Abstract: | Inside-out thylakoid vesicles have been separated from right-side-out material after press disruption of chloroplast lamellae. The separation was obtained by partition in an aqueous dextran-polyethylene glycol two-phase system, a method which utilizes differences in surface properties for separation of membrane particles. The isolated thylakoid vesicles showed the following inside-out properties: (1) light-induced reversible proton extrusion into the surrounding medium when supplied with the Photosystem II electron acceptor phenyl-p-benzoquinone; (2) a pH rise in the internal phase accompanying the external proton release, (3) sensitivity to trypsin treatment different from that of thylakoid membranes of normal orientation; (4) concave EF and convex PF freeze-fracture faces. |
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Keywords: | DCMU 3-(3,4-dichlorophenyl)1,1-dimethylurea DBMIB |
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