Incorporation of galactose from UDP-galactose into microsomal and golgi membranes of rat liver |
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Authors: | Eeva-Liisa Appelkvist Anders Bergman Gustav Dallner |
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Institution: | 1. Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, Stockholm Sweden;2. Department of Pathology at Huddinge Hospital, Karolinska Institutet, Stockholm Sweden |
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Abstract: | Rough and smooth microsomes and Golgi membranes were incubated with UDP14C]galactose and the incorporation of radioactivity into the lipid extract and into endogenous protein acceptors were measured. Antagonistic pyrophosphatases were inhibited with ATP and interference from β-galactosidase activity was greatly decreased by carrying out the incubation at pH 7.8. After incubation the particles were centrifuged to remove free oligosaccharide residues. Radioactivity was found in the lipid extract from Golgi membranes but not from rough and smooth microsomes. This radioactivity, however, was not associated with dolichol or retinyl phosphates. The incorporation of radioactivity into proteins of the Golgi fraction was more than double than that of the microsomal fractions. In addition, the transferases in these two types of particles exhibited different properties. Trypsin treatment of intact rough microsomal vesicles, smooth vesicles and Golgi membranes removed about 5, 15 and 50%, respectively, of newly incorporated protein-bound galactose, indicating that the proportion of the newly galactosylated proteins, which are localized at the cytoplasmic surface of the membrane, is lowest in rough microsomes, intermediate in smooth, and highest in Golgi membranes. |
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Keywords: | MOI average number of infecting phages per cell |
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