Specific release of plasma membrane enzymes by a phosphatidylinositol-specific phospholipase C |
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Authors: | Martin G Low JBryan Finean |
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Institution: | Department of Biochemistry, University of Birmingham, P.O. Box 363, Birmingham B15 2TT U.K. |
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Abstract: | The release of plasma membrane ecto-enzymes by a phosphatidylinositol-specific phospholipase C from Staphylococcus aureus was investigated. There was no effect on l-leucyl-β-naphthylamidase, alkaline phosphodiesterase I and Ca2+- or Mg2+-ATPase, but substantial proportions of the alkaline phosphatase and 5′-nucleotidase were released. There was no simultaneous release of phospholipid and the solubilized enzymes were not excluded from Sepharose 6-B. It was therefore concluded that release was not a secondary consequence of membrane vesiculation but occurred as a result of the disruption of specific interactions involving the phosphatidylinositol molecule. |
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Keywords: | HEPES |
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