Detection of progeny immune responses after intravenous administration of DNA vaccine to pregnant mice |
| |
Authors: | Ke-Qin Xin Shin Sasaki Yoshitsugu Kojima Nao Jounai Yasuko Kumamoto Kumiko Hashimoto Kaori Shinoda Kenji Hamajima Kenji Okuda |
| |
Institution: | (1) Department of Bacteriology, Yokohama City University School of Medicine, 236-0004 Yokohama, Japan;(2) Department of Bioregulation, Leprosy Research Center, National Institute of Infectious Diseases, 189-0002 Tokyo, Japan |
| |
Abstract: | A number of factors influence the development of tolerance, including the nature, concentration and mode of antigen presentation
to the immune system, as well as the age of the host. The studies were conducted to determine whether immunizing pregnant
mice with liposome-encapsulated DNA vaccines had an effect on the immune status of their offspring. Two different plasmids
(encoding antigens from HIV-1 and influenza virus) were administered intravenously to pregnant mice. At 9.5 days post conception
with cationic liposomes, injected plasmid was present in the tissues of the fetus, consistent with trans-placental transfer.
When the offspring of vaccinated dams were immunized with DNA vaccine, they mounted stronger antigen-specific immune responses
than controls and were protected against challenge by homologous influenza virus after vaccination. Moreover, such immune
responses were strong in the offspring of mothers injected with DNA plasmid 9.5 days after coitus. These results suggest that
DNA vaccinated mothers confer the antigen-specific immunity to their progeny. Here we describe the methods in detail as they
relate to our previously published work.
Published: April 23, 2002 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|