A simple method of DNA extraction from soil for detection of composite transgenic plants by PCR |
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| Authors: | Dieter Ernst Evi Kiefer Alain Drouet Heinrich Sandermann |
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| Institution: | 1. GSF-Institut für Biochemische Pflanzenpathologie, D-85758, Oberschlei?heim, Germany
2. Université d'Orléans, Laboratoire de Biologie des Ligneux Forestiers, BP6749 45067, Orléans Cedex 2, France
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| Abstract: | This new and simple method of DNA extraction from composite soil allows the isolation of plant DNA with high efficiency, quality
and reproductivity. The method is based on a simple CaCl2-precipitation step and requires no additional purification steps to eliminate humic acids. The extracted DNA was obtained
in sufficient purity and quantity to allow direct detection of transgenes by PCR. Furthermore, the simple procedure allows
the assay of many samples at the same time. |
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| Keywords: | DNA extraction PCR soil transgene |
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