In vitro selection at cellular level for red rot resistance in sugarcane (<Emphasis Type="Italic">Saccharum</Emphasis> sp.) |
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Authors: | A S Sengar K S Thind Bipen Kumar Mittal Pallavi S S Gosal |
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Institution: | (1) Department of Plant Breeding, Genetics and Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, 141 004, India |
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Abstract: | In the present study, in vitro selection technique using pathogen culture filtrate of Colletotrichum falcatum Went was employed with the aim to identify associations (if any), between selection at the cellular and plant level for red
rot resistance in sugarcane (Saccharum sp.). Five to eight months old sugarcane calli of genotypes CoJ 88 and CoJ 64 were screened in vitro against pathogen culture
filtrate for two selection cycles. Effect of pathogen culture filtrate on callus survival and/or proliferation was observed
to be directly related to its concentration in the selection media. Calli survived and exhibited further proliferation at
5, 10 and 15% v/v pathogen culture filtrate concentrations whereas, at higher concentrations (20 and 25% v/v) proliferation
was completely inhibited. Shoot regeneration percent was higher in calli selected on 5% pathogen culture filtrate concentration
than those selected on 10 and 15% concentrations. In vivo screening of field transferred somaclones against two pathtypes
(Cf 03 and Cf 08) showed considerable variation for red rot resistance. Somaclones regenerated from resistant and/or tolerant
calli exhibited better resistance than the parental genotypes. The results indicated that in vitro selection for red rot resistance
was effective and expressed when somaclones were screened in the field. This indicated a positive association between in vitro
and in vivo methods of selection for disease resistance in sugarcane. |
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Keywords: | Callus Pathogen culture filtrate In vitro Red rot Sugarcane |
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