Insertion of pea lectin into a phospholipid monolayer |
| |
| Authors: | Pettie Booij Rudy A. Demel B. Sylvia de Pater Jan W. Kijne |
| |
| Affiliation: | (1) Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, Netherlands;(2) RUL-TNO Center for Phytotechnology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, Netherlands;(3) Department of Biochemistry of Membranes, Centre for Biomembranes and Lipid Enzymology, University of Utrecht, Pddualaan 8, 3584 CH Utrecht, Netherlands |
| |
| Abstract: | Pea lectin (PSL) is a secretory sugar-binding protein, readily soluble in aqueous solutions of low osmolarity. However, PSL also appears to be associated with the plasma membrane at the tip of young pea root hairs. By using the Wilhelmy plate method, we found that PSL can insert into a lipid monolayer. This property appeared to be independent of the sugar-binding ability of the protein. This result suggests that PSL may be directly involved in membrane-mediated interactions with saccharide ligands, for example during root hair infection by symbiotic rhizobia. |
| |
| Keywords: | pea lectin phosphatidylcholine monolayer Wilhelmy plate method |
| 本文献已被 SpringerLink 等数据库收录！ |
|
