宁夏枸杞器官发生和体细胞胚胎发生过程中DNA、RNA和蛋白质合成动态的比较研究

宁夏枸杞(Lycium barbarum L.)叶外植体来源的愈伤组织经筛选、繁殖后,将来源相同、状态较为一致的淡黄色愈伤组织转移至O型或E型培养基上,可以诱导出器官发生和体细胞胚胎发生。利用该体系,对两条离体再生途径进行了比较研究。结果表明:(1)在拟分生组织和胚性细胞形成之前,RNA合成首先被激活,随后DNA、蛋白质合成加速;而球形胚形成期间,先是DNA合成的加快,接着RNA、蛋白质的合成高峰出现,在不定芽形成期间却正好相反;(2)可溶性蛋白组分发生规律性变化;器官发生和体细胞胚胎发生的启动阶段都有-153.6kD多肽出现,一些多肽分子在分化早期逐渐消失,而随芽原基或球形胚的形成又重新合成;与形态发生相对应,两种再生体系都有作为各自分子标记的特异多肽(84.9kD、46.3kD和44kD、36.2kD)的表达。此外,还对两种离体再生体系之间的关系和发生机制进行了讨论。

A COMPARATIVE STUDY ON THE SYNTHESES OF DNA.RNA AND PROTEIN DURING IN VITRO ORGANOGENESIS AND SOMATIC EMBRYOGENESIS OF LYCIUM BARBARUM L.

After the calli originating from the leaf ex-plant of Lycium barbarum L. were selected and proliferated, the yellowish calli with same origin, similar state were transferred to O medium or E medium and the regenerative systems of organogenesis and somatic embrogenesis might form. By these systems, a comparative study on the synthetic activities of DNA, RNA and protein in the two in vitro regeneration pathways was carried out. The results were as follows-(1 ) Before meristemoid and embryogenic cells were formed, the synthesis of RNA was activated firstly, followed with the increase of synthesis rates of DNA and protein. During the formation of globular embryo, the synthesis rate of DNA increased quickly and then the activities of syn- theses of RNA and protein reached the peak, while it was the contrary during germination of adventitious bud. (2) Components of soluble protein changed regularly. A peptide (153. 6kD) appeared during the initiation of both organogenesis and somatic embryogenesis. Several peptides disappearing gradually in the early-stage of differentiation could regenerate with the formation of shoot primordium and globular embryo. Corresponding to morphogenesis, both regenerative systems had specific peptides (84. 9kD, 46. 3kD and 44kD, 36. 2kD) as molecular markers of its own development. In addition, the relation and mechanism of the two regenerative systems were discussed.