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An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains |
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| Authors: | van Dijken JP Bauer Brambilla Duboc Francois Gancedo Giuseppin Heijnen Hoare Lange Madden Niederberger Nielsen Parrou Petit Porro Reuss van Riel N Rizzi Steensma Verrips Vindeløv Pronk |
| Affiliation: | a Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands b Nestlé Research Centre, PO Box 44, Vers-Chez-les-Blanc, 1000 Lausanne, Switzerland c Dipartimento di Fisiologia e Biochimica Generali, Universita Degli Studi di Milano, via Celoria 26, 20133 Milano, Italy d Department of Biotechnology, Denmarks Tekniske Universitet, Building 223, DK 2800 Lyngby, Denmark e Departement de Génie Biochimique et Alimentaire, Centre de Bioingénierie Gilbert Durand, Complexe Scientifique de Rangueil, 31077 Toulouse, France f Instituto Investigaciones Biomédicas, Arturo Duperier 4, 28029 Madrid, Spain g Unilever Research Laboratories, PO Box 114, 3133 AT Vlaardingen, The Netherlands h Department of Bioprocess Engineering, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands i Department of Chemical and Biochemical Engineering, University College London, Torrington Place, London WC1E 7 JE, United Kingdom j Institut für Bioverfahrenstechnik, Universität Stuttgart, Allmandring 31, 70569 Stuttgart, Germany k Institute for Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 AL Leiden The Netherlands |
| Abstract: | To select a Saccharomyces cerevisiae reference strain amenable to experimental techniques used in (molecular) genetic, physiological and biochemical engineering research, a variety of properties were studied in four diploid, prototrophic laboratory strains. The following parameters were investigated: 1) maximum specific growth rate in shake-flask cultures; 2) biomass yields on glucose during growth on defined media in batch cultures and steady-state chemostat cultures under controlled conditions with respect to pH and dissolved oxygen concentration; 3) the critical specific growth rate above which aerobic fermentation becomes apparent in glucose-limited accelerostat cultures; 4) sporulation and mating efficiency; and 5) transformation efficiency via the lithium-acetate, bicine, and electroporation methods. On the basis of physiological as well as genetic properties, strains from the CEN.PK family were selected as a platform for cell-factory research on the stoichiometry and kinetics of growth and product formation. |
| Keywords: | Yeast Strain choice Physiology Genetics Engineering |
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