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| 耙齿菌糖蛋白的提取分离、理化性质及抗炎活性 | |
| 引用本文： | 杨真威,姜瑞芝,陈英红,高其品. 耙齿菌糖蛋白的提取分离、理化性质及抗炎活性[J]. 天然产物研究与开发, 2005, 17(3): 280-282 |
| 作者姓名： | 杨真威 姜瑞芝 陈英红 高其品 |
| 作者单位： | 1. 吉林大学生命科学学院,长春,130023 2. 吉林省中医中药研究院,长春,130021 3. 吉林省药品检验所,长春,130062 |
| 摘 要： | 耙齿菌发酵物经水提醇沉得醇沉Ⅰ、醇溶Ⅱ两部分；Ⅰ透析得内液Ⅰ1、外液Ⅰ2。苯酚-硫酸法、Lowry法、间羟基联苯法测Ⅰ的总糖、蛋白质、糖醛酸的含量分别为43．22、21．11、4．32％；除蛋白和氨基酸分析证明Ⅰ为糖蛋白。气相色谱测定Ⅰ的组成糖及摩尔比为Ara：Xyl:Man：Gal：Glu=1：0．5：4．2：1．6：6．1；HPLC测定分子量为60kD；小鼠耳肿胀实验证实Ⅰ具有抗炎活性。 |
| 关 键 词： | 抗炎活性 糖蛋白 提取分离 理化性质 耙齿 苯酚-硫酸法 Lowry法 气相色谱测定 HPLC测定 氨基酸分析 发酵物 间羟基 蛋白质 糖醛酸 Gal Ara 摩尔比 分子量 醇沉 小鼠 |
| 修稿时间： | 2004-05-28 |
The Extraction, Isolation, Physicochemical Properties and Anti-inflammation Activity of Irpex Lacteus Fr. |
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| YANG Zhen-Wei,JIANG Rui-zhi,CHEN Ying-hong,GAO Qi-pin. The Extraction, Isolation, Physicochemical Properties and Anti-inflammation Activity of Irpex Lacteus Fr.[J]. Natural Product Research and Development, 2005, 17(3): 280-282 | |
| Authors: | YANG Zhen-Wei JIANG Rui-zhi CHEN Ying-hong GAO Qi-pin |
| Affiliation: | YANG Zhen-wei 1,JIANG Rui-zhi 2,CHEN Ying-hong 2,GAO Qi-pin 3* |
| Abstract: | Irpex Lacteus Fr. was soluted with water and deposited with ethanol. Two parts, precipitate I and supernatant II were obtained; I was dialysed against water, I_1 and I_2 were obtained. Total carbohydrates, protein, uronic acids of I were assayed by the phenol-H_2SO_4, Lowry, and m-hydroxybiphenyl,and the contents were 43.2, 21.11, 4.32%, respectively; by getting rid of proteins and amino acid analysis I was identified as a glycoprotein. GC and HPLC were used to determine the component sugars and molecular weight, I was composed of Ara?Xyl?Man?Gal?Glu and molar ratios were 1:0.5:4.2:1.6:6.1, and molecular weight of I was 60 kD. The swelling experiment of mouse ear induced by xylene confirmed the anti-inflammation activity of I. |
| Keywords: | Irpex Lacteus Fr. glycoprotein anti-inflammation activity |
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