Immunohistochemical localization of xenogeneic antibodies against Iak lymphocytes on B cells and reticular cells |
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Authors: | Gertrud Hoffmann-Fezer Dietrich Götze Hans Rodt Stefan Thierfelder |
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Institution: | (1) Institut für Hämatologie, Landwehrstraße 61, Munich, Germany;(2) Present address: The Wistar Institute, Philadelphia, Pennsylvania |
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Abstract: | Rabbit antiserum against B10.AQR mouse spleen and lymph node cells (RAQR), after appropriate absorption, reacted with Iak-positive spleen and lymph node cells in cytotoxic and complement-fixing indicator systems. It reacted neither with Iak-positive thymocytes nor Iak-negative thymocytes, spleen, and lymph node cells. Cryostat sections of tissue from Iak-positive and Iak-negative mice were incubated with RAQR and either rabbit anti-mouse Ig or rabbit anti-T cell globulin. With the unlabeled antibody enzyme method, RAQR-stained lymphocytes were concentrated in the B-cell regions of spleen and lymph nodes of Iak-positive CBA mice. The tissues of mice bearingI-region haplotypes different fromk were negative. Reticular cells of the T cell-supporting network were also positive in Iak mice, but liver, gall bladder, and testicular cells were not. Macrophages of both Iak-positive and -negative mice were stained by RAQR and also by heat-aggregated, peroxidase-labeled Ig. Iak-positive reticular cells survived 900 R total body irradiation and persisted after grafting with Iak-negative bone marrow. The reticular cells were also seen in a thymus which was depleted of cortisone-sensitive lymphocytes.Abbreviations used in this paper are as follows RAQR
rabbit anti-mouse-B10.AQR globulin
- RAMTG
rabbit anti-mouse T-cell globulin
- RAMIG
rabbit anti-mouse Ig
- SARIG
sheep anti-rabbit Ig
- agg-HIg
aggregated human Ig
- PAP
anti-peroxidase-peroxidase-complex |
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