| EB荧光分析法测定肿瘤细胞DNA交联及增殖活性 |
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| 引用本文： | 黄建鸣,董玉宁,许峰,文浩,王瑾,郎锦义. EB荧光分析法测定肿瘤细胞DNA交联及增殖活性[J]. 生物化学与生物物理进展, 1996, 23(1): 70-73 |
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| 作者姓名： | 黄建鸣 董玉宁 许峰 文浩 王瑾 郎锦义 |
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| 作者单位： | 四川省肿瘤研究所;四川省肿瘤研究所;四川省肿瘤医院放疗科;四川省肿瘤医院放疗科;四川省肿瘤医院放疗科;四川省肿瘤医院放疗科 |
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| 基金项目： | 四川省卫生厅资助项目. |
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| 摘 要： | 应用EB荧光分析法测定体外培养人宫颈癌细胞株(HeLa)、人白血病细胞株(HL-60)，增殖性和非增殖性人外周血淋巴细胞(PBL)的DNA含量及其交联度(DNA cross-link)，并据此研究不同增殖状态细胞与其DNA百分交联度(ct%)的相互关系.结果显示，HeLa细胞、HL-60细胞、增殖性和非增殖性PBL的DNA ct%分别为36.5、 22.5、 20.2和0，表明不同增殖速度或周期的细胞具有不同的DNA交联反应，而非增殖性细胞或G0期细胞不产生DNA交联反应.
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| 关 键 词： | 恶性肿瘤 细胞株 DNA交联反应 细胞增殖活性 EB荧光分析法 |
| 收稿时间： | 1995-01-25 |
| 修稿时间： | 1995-05-13 |
Detection of DNA Cross-link and Proliferative Activity in Tumor Cell with EB-Fluorescence Assay |
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| Huang Jianming,Dong Yuning,Xu Feng,Wen Hao,Wang Jin and Lang Jinyi. Detection of DNA Cross-link and Proliferative Activity in Tumor Cell with EB-Fluorescence Assay[J]. Progress In Biochemistry and Biophysics, 1996, 23(1): 70-73 |
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| Authors: | Huang Jianming Dong Yuning Xu Feng Wen Hao Wang Jin Lang Jinyi |
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| Affiliation: | Department of Biochemistry, Sichuan Cancer Institute;Department of Biochemistry, Sichuan Cancer Institute;Sichuan Cancer Hospital;Sichuan Cancer Hospital;Sichuan Cancer Hospital;Sichuan Cancer Hospital |
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| Abstract: | The detection of DNA contents and ds-DNA cross-link in HeLa line, HL-60 line,proliferative and non-proliferative T lymphocytes with EB-fluorescence assay was investigated, and study on the correlation of cell proliferative activity with its ds-DNA cross-link was carried out as well. The results show that the percentages of ds-DNA cross-link in HeLa, HL-60 line, proliferative and nonproliferative T lymphocytes were 36. 5, 22. 2,20. 2 and 0 respectively, and that the proliferative activity of cells has a relationship with their ds-DNA cross-link, and nonproliferative or Go phase cells do not produce ds-DNA cross-link. |
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| Keywords: | malignant tumor cell line DNA cross-link proliferative activity of cell EB-fluorescence assay |
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