| 表面等离子体激元共振与生物分子相互作用分析 |
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| 引用本文： | 汪洛,陶祖莱. 表面等离子体激元共振与生物分子相互作用分析[J]. 生物化学与生物物理进展, 1996, 23(6): 483-487 |
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| 作者姓名： | 汪洛 陶祖莱 |
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| 作者单位： | 中国科学院动物研究所, 生物膜与膜生物工程国家重点实验室;中国科学院力学研究所 |
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| 摘 要： | 对表面等离子体激元共振（surface plasmon resonance, SPR）的原理和在生物学研究方面的应用进行了综述.这种技术可以直接原位、实时地跟踪生物学实验研究系统，而不需要附加参数如进行标记等手段，具有高敏感性，也可以连续监测吸附或解吸附过程，目前有关的应用涉及到生物学结合分析、动力学及亲和力测定、免疫识别研究、结构与活性研究和核酸研究等多个领域.
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| 关 键 词： | 表面等离子体共振 生物分子相互作用 实时分析 感应片表面 |
| 收稿时间： | 1996-01-23 |
| 修稿时间： | 1996-04-01 |
Surface Plasmon Resonance and Biomolecular Interaction Analysis |
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| Wang Luo and Tao Zulai. Surface Plasmon Resonance and Biomolecular Interaction Analysis[J]. Progress In Biochemistry and Biophysics, 1996, 23(6): 483-487 |
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| Authors: | Wang Luo and Tao Zulai |
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| Affiliation: | State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Academia Sinica;Laboratory of Biological Dynamics, Instituteof Mechanics, Academia Sinica |
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| Abstract: | The principle of surface plasmon resonance and the applications of this theory,particularly in biological fields are reviewed.Using this technique,the experimental system can be directly probed and followed in real time and in situ without the requirement for additional parameters, such as labelled moleculars.With high sensitivity,the sequential reactions during adsorption or desorption can be monitored. So far, the applications are involved in biological binding analysis, kinetics, affinity measurement, immuno-distinguish, structure-actmty study and nucleic acid research, etc. |
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| Keywords: | surface plasmon resonance biomolecular interaction real time analysis sensor chip surface |
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